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2017-2018 LAB Microbial Soil

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LAB

Microbial

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E. coli
  • Legend
  • E. coli
E. coli
E. coli
Contaminant Name: 
E. coli
Contaminant Type: 
Bacterium
What is E. coli?
E. coli stands for Escherichia coli. Escherichia coli are bacteria naturally found in the feces (poop) of warm- and cold-blooded animals (including humans). Because animals have been defecating in the environment for centuries, E. coli are commonly detected in environmental samples. Although they are commonly associated with foodborne illness, most types of E. coli are harmless and can serve as bacteria to indicate if something has been contaminated with feces.

What happens when E. coli enters the environment?
E.coli found in water can indicate when it has been contaminated with fecal matter.

How can E. coli affect my health? As previously stated, most E. coli are not pathogenic, but toxin-producing E. coli can cause diarrhea, urinary tract infections, pneumonia, and other illnesses.

Where can I get more information on E. coli?
  • Centers for Disease Control. E.coli (Escherichia coli). Last Updated on February 26, 2018
    https://www.cdc.gov/ecoli/general/index.html
  • US EPA Fecal Bacteria. Last Updated on March 06, 2012
    archive.epa.gov/water/archive/web/html/vms511.html

Important note: The use of SRB for assessment of contamination is very unique to this project. Though preliminary research suggests that SRB may be a strong indicator of fecal contamination, this has not been tested in harvested rainwater or in the American Southwest. In the third year of the project, we will compare the number of E. coli and Total Coliforms (for which there are standards) to the number of SRB (for which there are no standards) in each sample. We hope to discover that homeowners will be able to use a kit that identifies a non-pathogenic organism (SRB) to assess the quality of their harvested rainwater. This would be very exciting, given that at-home kits for microbial water quality assessment do not exist.

Results shown below the Method Limit of Detection (MLOD) were measured at a time when the instrument's ability to detect was better, which provided a lower MLOD than shown here.

Salmonella
  • Legend
  • Salmonella
Salmonella
Salmonella
Contaminant Name: 
Salmonella
Contaminant Type: 
Bacteria
What is Salmonella
Salmonella are bacteria that can be found in the intestines of many animals; however, it is most common in reptiles, birds, and amphibians. Swine and ruminant animals can also be carriers of the bacteria. The bacteria are introduced into the environment through feces, and the most common mode of transmission is a feces-to-oral pathway. Chickens are a common source of Salmonella, as infected hens lay eggs infected with the bacterium. Nearly all strains of Salmonella are pathogenic, causing an infection called salmonellosis.

What happens when Salmonella enters the environment?
The presence of Salmonella can indicate fecal contamination. The contamination does not have to be recent, however, as Salmonella can survive in soil for weeks.

How can Salmonella affect my health?
Salmonella can cause an infection called salmonellosis. The symptoms usually include diarrhea, fever, vomiting, and abdominal cramps lasting up to a week.

Where can I get more information on Salmonella?
  • Centers for Disease Control. Salmonella. Last Updated on February 21, 2019
    www.cdc.gov/salmonella/index.html
  • Food Safety. US Department of Health and Human Services. Salmonella. n.d
    www.foodsafety.gov/poisoning/causes/bacteriaviruses/salmonella/index.html

Important note: The use of SRB for assessment of contamination is very unique to this project. Though preliminary research suggests that SRB may be a strong indicator of fecal contamination, this has not been tested in harvested rainwater or in the American Southwest. In the third year of the project, we will compare the number of E. coli and Total Coliforms (for which there are standards) to the number of SRB (for which there are no standards) in each sample. We hope to discover that homeowners will be able to use a kit that identifies a non-pathogenic organism (SRB) to assess the quality of their harvested rainwater. This would be very exciting, given that at-home kits for microbial water quality assessment do not exist.

Results shown below the Method Limit of Detection (MLOD) were measured at a time when the instrument's ability to detect was better, which provided a lower MLOD than shown here.

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Results

lab
inorganic water soil plant
microbial water soil
organic water
diy
inorganic water soil
microbial water

Materials

Contaminants and Standards and/or Reference Values Descriptions for Water, Soil, and Plants
Project Overview
Important Terms/ References
How to read your results
*The results have been internally reviewed but have not undergone external peer review and may be subjected to minor changes.
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This material is based upon work supported by the National Science Foundation under Grant No. DRL-1612554. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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